Identification of new polymorphic microsatellite markers in the NA1 and NA2 lineages of Phytophthora ramorum.

Author , ,
Date 2011.
Publication Mycologia.
Key Words
DOI10.3852/10-420.
AbstractPhytophthora ramorum is a recently introduced pathogen in Europe and North America consisting of three clonal lineages. Due to the very limited intra-lineage genetic variation, only a few polymorphic markers are available for use in studies involving the epidemiology and evolution of P. ramorum. A total of 159 primer pairs for candidate polymorphic SSR loci were tested with universal labeling. Four polymorphic microsatellite loci were identified within the NA1 lineage and one within the NA2 lineage, demonstrating the power and flexibility of the screening technique. The markers may significantly increase the number of genotypes that can be identified, and as such can help better characterize the North American lineages of P. ramorum.
Full Citation Vercauteren, A.; Larsen, M.; Goss, E.; Grunwald, N.; Maes, M.; and Heungens, K. 2011. Identification of new polymorphic microsatellite markers in the NA1 and NA2 lineages of Phytophthora ramorum. Mycologia. DOI: 10.3852/10-420.