First report of Phytophthora ramorum infecting Trachelospermum jasminoides in Oregon.

Author , , ,
Date 2011.
Publication Phytopathology 101:S134.
Key Words
AbstractPhytophthora ramorum can reportedly infect >125 plant species, many of which move through the nursery trade. In April 2010, P. ramorum was detected infecting Camellia plants growing in a nursery in Washington County, Oregon. During the delimitation survey to determine the extent of the infestation, necrotic leaf spots and premature leaf abscission were observed on several plants of star jasmine (Trachelospermum jasminoides). Five symptomatic leaves were collected for testing using a commercial Phytophthora DAS-ELISA kit (AgDia Inc., Elkhart, IN). DNA was extracted from ELISA-positive tissue using a Plant DNeasy kit (Qiagen Inc., Valencia, CA) and then tested for P. ramorum using the federally approved ITS and Elicitin qPCR protocols (USDA/APHIS/PPQ/CPHST Work Instructions WI-B-T-1-6 and WI-B-T-1-7). Isolations were made from the symptomatic leaves onto the semi-selective medium PARP. One Phytophthora isolate was obtained from the symptomatic leaves; this isolate was morphologically identical to P. ramorum. Results from the ITS and Elicitin qPCR tests verified the presence of P. ramorum DNA in the leaf tissue. As required by federal protocol, a subsample of DNA was sent to the USDA/APHIS/PPQ Molecular Diagnostic Laboratory for official confirmation. In June 2010, USDA/APHIS/PPQ/MDL confirmed our identification of P. ramorum. To our knowledge, this is the first report of P. ramorum infecting star jasmine in Oregon.
Full Citation Osterbauer, N.; Trippe, A.; Lane, S.; and S. Lewis, S. 2011. First report of Phytophthora ramorum infecting Trachelospermum jasminoides in Oregon. Phytopathology 101:S134.