Phytophthora species identified from streams in Virginia.

Author , , , , ,
Date 2011.
Publication Phytopathology 101:S74.
Key Words
AbstractThe genus Phytophthora was added to the list of ‘water molds’ in 1944 and this notion has since been generally accepted. However, little is known about its aquatic biology. Here we report the species of Phytophthora recovered during a statewide survey of streams for Phytophthora ramorum from 2006 to 2010. Rhododendron leaves were used as baits and placed in streams for 1 to 2 weeks, depending on the temperature. A total of 56 streams were baited each for five to six times from April to October. Leaves retrieved from water were wrapped in moist paper towels and shipped overnight to the lab, rinsed in deionized water then plated in 10-cm Petri dishes containing PARPH V8 agar. Emerging colonies were identified to species level by colony PCR-SSCP, morphology and DNA sequence analysis. A total of 1372 isolates were examined and 415 subcultured. Seven species of Phytophthora were recovered from 7 streams in 2006, six from 10 streams in 2007, 15 from 20 streams in 2008, 13 from 19 streams in 2009 and nine from 10 streams in 2010, respectively. By stream, the minimum number of species isolated was 1 and the maximum was 9. Fifteen species and several new taxa were recovered across all streams. These include P. taxon ‘aquatilis’, P. cambivora, P. citrophthora, P. cryptogea, P. gonapodyides, P. hydropathica, P. insolita, P. irrigata, P. megasperma, P. parsiana, P. pini, P. plurivora, P. pseudosyringae, P. sansomeana, and P. tropicalis.
Full Citation Hong, C.; Richardson, P.; Kong, P.; Edgerton, T.; Asaro, C.; and Oak, S. 2011. Phytophthora species identified from streams in Virginia. Phytopathology 101:S74.